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VetLine Brucella Ab, Elisa kit

VetLine Brucella Ab, Elisa kit

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96 determinations for Brucella Ab in veterinary serum, pooled serum samples and milk samples.

Article number: BRUVT0050
Content: Kit (96 Wells)
Storage: ❄️ Refrigerated (2-8°C)

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Brand: VetLine

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The NovaTec VetLine Brucella ELISA is intended for the qualitative determination of antibodies against Brucella in veterinary serum, pooled serum samples and milk samples.
The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique.
Microtiterplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product.
The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA Microtiterplate reader.

Reagents supplied
  • Microtiterplate: 12 break-apart 8-well snap-off strips coated with Borrelia antigens; in resealable aluminium foil.
  • Sample Dilution Buffer: 1 bottle containing 100 mL of phosphate buffer (10 mM) for sample dilution; pH 7.2 ± 0.2; coloured yellow; ready to use; white cap; ≤ 0.0015 % (v/v) CMIT/MIT (3:1).
  • Stop Solution: 1 bottle containing 15 mL sulphuric acid, 0.2 mol/L; ready to use; red cap.
  • Washing Buffer (20x conc.): 1 bottle containing 50 mL of a 20-fold concentrated phosphate buffer (0.2 M), pH 7.2 ± 0.2, for washing the wells; white cap.
  • Conjugate: 1 bottle containing 20 mL of peroxidase labelled Protein A/G; coloured yellow; ready to use; white cap; ≤ 0.02 % (v/v) MIT.
  • TMB Substrate Solution: 1 bottle containing 15 mL 3,3',5,5'-tetramethylbenzidine (TMB), < 0.1 %; ready to use; yellow cap.
  • Positive Control: 1 vial containing 2 mL; coloured yellow; ready to use; red cap; ≤ 0.02 % (v/v) MIT.
  • Cut-off Control: 1 vial containing 3 mL; coloured yellow; ready to use; green cap; ≤ 0.02 % (v/v) MIT.
  • Negative Control: 1 vial containing 2 mL; coloured yellow; ready to use; blue cap; ≤ 0.0015 % (v/v) CMIT/MIT (3:1).