Theileria equi Antibody Test Kit, cELISA Kit

This Theileria equi Antibody Test Kit is a competitive, enzyme-linked, immunosorbent assay (cELISA). Sample serum T. equi antibody inhibits binding of primary monoclonal antibody. The binding of primary monoclonal antibody to the antigen-coated plate is detected with HRP-labeled secondary antibody. Finally, the presence of HRP-labeled secondary antibody is quantified by the addition of enzyme substrate and subsequent color product development. Strong color development indicates little or no inhibition of primary monoclonal antibody binding and therefore the absence of T. equi antibody in sample sera. Weak color development due to inhibition of the primary monoclonal antibody binding to the antigen on the solid phase indicates the presence of T. equi antibodies in sample sera.